Figure 5.

(A) Signals of EMRS for let-7d in total RNAs (5.0 μg) extracted from liver, lung and prostate tissues. Synthetic let-7d (1 pM) was used as positive control and the negative control was the signal obtained in the absence of total RNAs. (B) Detection of let-7d with EMRS using different amount of total RNAs from prostate tumoral tissue and normal tissue. (C) Detection of miR-21 with EMRS in the total RNA of esophageal squamous cell carcinoma (ESCC) patients (all of 100 ng). Statistical analysis was performed by paired t-test. * P < 0.05 significantly different from relative normal tissue. Error bars represent standard deviations for measurements taken from at least three independent experiments.