Figure 3.

Spatial organization of the WGA-immunoreactive cells in the paraventricular nucleus. (A–D) Nissl-stained cross sections through the paraventricular nucleus (PVN). The third ventricle (3V) is indicated by the continuous line. The dashed lines mark the extent of the paraventricular nucleus and separate the periventricular part (pv) from the major portion (mp) of the nucleus. These divisions are transferred to the respective neighboring sections shown in (A′–D′). (A′–D′) All along the anterior–posterior axis of the paraventricular nucleus, no WGA-immunoreactivity can be observed in the periventricular part. WGA-immunoreactive cells are located in the major portion of the nucleus. In the rostral part these cells are clustered in the ventromedial subdivision, at more caudal levels the WGA-immunoreactivity is predominantly located in dorsolateral parts. All pictures represent wide field images. The spacing between the sections from different areas along the anterior-posterior axis is approximately 60–96 μm, respectively. Scale bars: 50 μm.