Table 1.

Targeted exons, sequences of the primers used for PCR amplification, amplicon length and PCR conditions for each investigated gene.

Gene	Targeted exons(s)	Forward primer (5′ –> 3′)	Reverse primer (5′ –> 3′)	Size (bp)	PCR conditions
BRAF	14 and 15	GCACAGGGCATGGATTACTT	GATGACTTCTGGTGCCATCC	194	Std but Tm at 55°C
NRAS	3	CGCACTGACAATCCAGCTAA	TCGCTTAATCTGCTCCCTGT	255	Std
HRAS	3	GGAAGCAGGTGGTCATTGAT	ACGTCATCCGAGTCCTTCAC	204	Std
KRAS	3	AGAGAGGCCTGCTGAAAATG	TTGACCTGCTGTGTCGAGAA	200	Std
TP53-1	2, 3, 4, 5	GTGACACGCTTCCCTGGAT	ACACGCAAATTTCCTTCCAC	658	Std
TP53-2	5, 6, 7	CCCTTCCCAGAAAACCTACC	AGCTGTTCCGTCCCAGTAGA	518	Std
TP53-3	6, 7, 8, 9, 10, 11	GCTGCTCAGATAGCGATGGT	GTGGGAGGCTGTCAGTGG	660	Std
PI3KCA-1	9, 10, 11	TGACTGGTTCAGCAGTGTGG	GGCCAATCTTTTACCAAGCA	341	Std
PI3KCA-2	20, 21	TTTTGACACAGGATTTCTTAATAGTGA	GGTCTTTGCCTGCTGAGAGT	418	Std but Tm at 55°C
PAX8/PPARγ	Pax8: 8, 9, 10 PPARγ: 3 8, 9 8, 10 8, 9	GCAACCTCTCGACTCACCAG (PAX8)	CATTACGGAGAGATCCACGG (PPARγ)	407 305 217 108	Std
RET/PTC1	RET:12, 13 H4: 1	GGCACTGCAGGAGGAGAAC (H4)	GATGACATGTGGGTGGTTGA (RET)	277	Std
RET/PTC3	RET:12, 13 ELE1: 7	AAGCAAACCTGCCAGTGG (ELE1)	TGCTTCAGGACGTTGAAC (RET)	240	Std but with 30 cycles
PTEN-1	4, 5, 6	GACATTATGACACCGCCAAA	CGCCACTGAACATTGGAATA	405	Std
PTEN-2	6, 7, 8, 9	GCTACCTGTTAAAGAATCATCTGGA	TGACGGCTCCTCTACTGTTTT	530	Std but Tm at 48°C
PBGD	11, 12, and 13	AAGGACCAGGACATCTTGGA	AACTGTGGGTCATCCTCAGG	266	Std but Tm at 55°C

Std: Standard conditions: 94°C for 5 min followed by 35 cycles consisting of incubations at 94°C for 30 s, 60°C (Tm) for 1 min and 72°C for 1 min. At the end of cycles the PCR mixtures were incubated at 72°C for 10 min. PBGD gene was used as a positive control for amplification. For each PCR, a negative control (the amplification mix without DNA) was used to detect possible contamination.