Table II.
Activity of RG-galacturonohydrolase toward structurally different RG fragments
| Oligosaccharide | Activity | GalA Released from Total in 45 h |
|---|---|---|
| units mg−1 | % | |
| 1:R-GA-R-GA | 0.81 | 1.2 |
| ↑ ↑ | ||
| G G | ||
| 3:R-GA-R-GA | 8.1 | 13 |
| 5:GA-R-GA | 28 | 47 |
RG-galacturonohydrolase (0.42 μg mg−1 substrate) was incubated with 0.025% (w/v) substrate solutions in 50 mm NaOAc buffer (pH 5.0), for 1 and 45 h at 40°C. Incubation mixtures and blanks were analyzed on HPAEC (gradient C). Explanation of symbols is given in Table I.