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. 2012 Sep 24;4(9):1830–1843. doi: 10.3390/v4091830

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

pZAC replicates faster in PBMC than pcMJ4. Growth kinetics of subtype B and C viruses on PBMCs. Infectious viruses (MOI: 0.05) were cultured for 8 days on PBMCs. The supernatants were titrated on TZM-bl cells to determine the viral titer as indicated. Experiments were done in triplicate. NL4-3 had the highest replication capacity, whereas pMJ4/ZACenv and pZAC replicated better than pcMJ4. pZAC and pcZAC has similar growth kinetics, whereas pMJ4 failed to show significant viral titers after 8 days of culture.