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Purification of STS from seeds of V. angularis. A, HIC of proteins on Phenyl Sepharose HP after protamine sulfate treatment and ammonium sulfate fractionation (dashed line, 1.0–0.0 m ammonium sulfate). B, Native preparative PAGE of pooled fractions from the HIC on a Bio-Rad PrepCell 491. C, Final purification by AEC on Resource Q (dashed line, 0.0–0.3 m NaCl).
