Figure 3.

Analysis of STS at various stages of purification. A, SDS-PAGE. Lane 1, Crude protein extract; lane 2, 35 to 55% ammonium sulfate precipitate after protamine sulfate treatment; lane 3, pooled fractions after chromatography on Phenyl Sepharose HP; lane 4, molecular mass markers; lane 5, pooled fractions after native PAGE; and lane 6, pooled fractions after chromatography on Resource Q. B, IEF of purified STS. Lanes were loaded with 0.1 to 1.0 μg of protein and stained with silver nitrate.