Mutual inhibition of STS and GOS activity. The
reaction mixtures contained 10 mm galactinol, 0 to 50
mm raffinose, and 0 to 20 mm ononitol. Assays
were analyzed for both stachyose and galactosylononitol. Data were
plotted as follows: A, Formation of stachyose at different
concentrations of ononitol with raffinose as the variable substrate.
Ononitol concentrations were: 1, 0 mm; 2, 2.9
mm; 3, 4 mm; 4, 6.7 mm; and 5, 20
mm. B, Formation of galactosylononitol at different
concentrations of raffinose with ononitol as the variable substrate.
The raffinose concentrations were: 1, 0 mm; 2, 7.1
mm; 3, 10 mm; 4, 16.7 mm; and 5, 50
mm.