Table III.
Substrate specificity of STS
| Galactosyl Acceptor | Concentration | Activity |
|---|---|---|
| mm | % | |
| Raffinose | 50 | 100.0a |
| Raffinose (assayed with 10 mm galactosylononitol) | 50 | 37.8 |
| Stachyose | 50 | n.d.b |
| Suc | 50 | n.d. |
| myo-Inositolc | 20 | 224.8 |
| 1d-4-O-Methyl-myo-inositol (d-ononitol) | 20 | 103.5 |
| 5-O-Methyl-myo-inositol (sequoyitol) | 20 | 126.9 |
| 1d-1-O-Methyl-myo-inositol (d-bornesitol) | 20 | n.d. |
| 1l-1-O-Methyl-myo-inositol (l-bornesitol) | 20 | n.d. |
| scyllo-Inositol | 20 | 16.9 |
| 1-O-Methyl-scyllo-inositol | 20 | 4.0 |
| d-chiro-Inositol | 20 | n.d. |
| 1d-3-O-Methyl-chiro-inositol (d-pinitol)d | 20 | 0.9 |
| l-chiro-Inositol | 20 | n.d. |
| 1l-2-O-Methyl-chiro-inositol (l-quebrachitol) | 20 | n.d. |
| muco-Inositol | 20 | n.d. |
| 1d-1-O-Methyl-muco-inositol | 20 | n.d. |
| epi-Inositol | 20 | 23.2 |
Reactions were assayed with 10 mm galactinol unless otherwise indicated.
a
Corresponds to an activity of 56 pkat per assay.
b
n.d., Not detected.
c
Assayed with 20 mm myo-[3H]inositol (10 MBq mmol−1). Reaction products were separated by HPLC on a Carbopac MA1 and monitored with a radiodetector.
d
The product of this reaction was identified as galactopinitol A.