Table 1.

Immuno-gold labeling of BCL-2 family proteins.

Fig. 3 panel	Sample source	Antibody used for immuno-gold labeling	Refs.
Evidence for OUTER mitochondrial membrane labeling
A	BAX transfected Cos-7 cells	Anti-Bax	[91]
B	SU-DHL-4 cells	Anti-Bcl-2 monoclonal MAb #100	Fig. 4 of [92]
C	Human lymph node biopsy/ lymphoma	Monoclonal mouse anti-human Bcl-2 (Dako 124)	Fig. 1D of [93]
D	Human lymph node biopsy/ lymphoma	Monoclonal mouse anti-human Bcl-2 (Dako 124)	Fig. 1E of [93]
E	Human lymph node biopsy/ lymphoma	Monoclonal mouse anti-human Bcl-2 (Dako 124)	Fig. 1C of [93]
F	FDC-P1 myeloid cells +bcl-2/neo virus	Monoclonal antibody Bcl-2–100	Fig. 3A of [94]
Evidence for INNER mitochondrial membrane labeling
G	HL60 (human promyelocytic leukemia)	Anti-BCL-2 “pAb” (rabbit anti-human BCL-2 polyclonal against amino acids 4–21 of human/mouse/rat on 10 nm gold)	Fig. 2B of [84]
H	Mouse brain (hippocampus)	Rabbit anti-BCL-xL (Biocarta)	[33]
I	HeLa (human epithelial cancer) stably expressing human BCL-2	Anti-BCL-2ΔC21 “mAb” (Santa Cruz) where 60% of mitochondrial label is inside mitochondria	[86]
J	HL60	Anti-BCL-2 “pAb” (10 nm gold) co-labeled with anti-monoamine oxidase (20 nm gold, black arrows) to mark outer mitochondrial membrane	Fig. 3A of [84]
K	Rat liver	Rabbit anti-human BCL-2 polyclonal antibody “pAb” (10 nm gold), which is enriched in mitochondria rather than ER	Fig. 2A of [84]
L	Rat brain	Rabbit anti-BCL-xL (Biocarta, 10 nm gold, 5 nm gold, white arrows), co-labeled with and with anti-SOD (5 nm gold)	[34]
M	Isolated PC12 mitochondria	Anti-Bcl-2 [anti-rat Bcl-2, PharMingen], cryothin-section immunogold	Fig. 4B of [85]
N	Mouse brain (hippocampus)	Rabbit anti-BCL-xL (Biocarta)	[33]
O	bcl-x conditional knockout mouse brain (hippocampus)	Rabbit anti-BCL-xL (Biocarta) prepared in parallel with panels H and N	[33]