Figure 4.
Effects of bFGF on osteogenic/dentinogenic gene expression after differentiation induction. SCAP at passage 3 never exposed to bFGF were cultured with osteogenic/dentinogenic induction medium with or without the presence of bFGF (5 ng/mL) for 1, 2, and 4 weeks. Expression of DSPP and OCN was decreased at 1 week (A), expression of ALP, BSP, and OCN was reduced at 2 weeks (B) when bFGF was present in the cultures. No significant change of expression of ALP, DSPP, OCN, and BSP was detected at 4 weeks (C). Bar charts represent the relative expression levels of each gene normalized against GADPH (*P < .05).