Fig. 3.
A). The flow cytometry histogram displays the relative fluorescence intensity of untreated controls in the dark (blue) or light (red) or JH-EsoAd1 cells treated with EDANS-Dox in the dark (orange) or light (green). B) Quantification of Dox fluorescence for the indicated treatment conditions. Data are representative of two independent experiments, N=9. C) Representative confocal images of JHEsoAd1 cells treated with EDANS-Dox in the dark or light. The red channel shows Dox fluorescence, the gray channel is a DIC image, and the overlay represents the cellular localization of Dox fluorescence.