PCP and LHC mRNA levels are increased under
reduced light conditions. A, Northern analysis of mRNA (2 μg/lane)
from A. carterae grown under various light conditions
and probed with either the insert in pRGH201 (Hiller et al., 1995) for
LHC or the part of the MFPCP gene encoding the mature protein
(generated by PCR; see figure 6A of Sharples et al., 1996). B, Slot
blots of mRNA from A. carterae grown under various light
conditions (High, moderate [Mod.], or Low) probed with probes for PCP
or LHC (as described in A) or for the housekeeping genes
ub52 and EF1α. Numbers to the right of
the blots are the corresponding standardized photon equivalents,
determined by quantitative phosphor imaging.