Table 1.
Contribution of background activities of CDH IIA and ascorbic acid, respectively, to the total activity measured with various concentrations of PMO (PMO-02916)
| Assay |
PMO concentration |
H2O2production |
PMO activity |
Background activity |
|---|---|---|---|---|
| [mg mL-1] | [μmol L-1min-1] | [μmol L-1min-1] | [%] | |
|
Ascorbate |
2.87 |
5.39 ± 0.23 |
4.90 |
9.1 |
| |
1.43 |
3.61 ± 0.22 |
3.12 |
13 |
| |
0.72 |
2.34 ± 0.05 |
1.85 |
21 |
| |
0.18 |
1.05 ± 0.01 |
0.56 |
47 |
| |
0.02 |
0.63 ± 0.02 |
0.14 |
78 |
| Blank |
0 |
0.49 ± 0.02 |
0 |
0 |
|
CDH IIA |
5.74 |
6.21 ± 0.22 |
5.51 |
11 |
| |
1.43 |
2.57 ± 0.11 |
1.87 |
27 |
| |
0.72 |
1.77 ± 0.11 |
1.07 |
40 |
| |
0.18 |
0.94 ± 0.05 |
0.24 |
74 |
| |
0.02 |
0.74 ± 0.05 |
0.04 |
94 |
| Blank | 0 | 0.70 ± 0.04 | 0 | 0 |