Table 3.
Purification scheme of recombinant PMO enzymes
| Purification step | Total proteina[g] | Total activity [U] | Specific Activity [U g-1] | Purification [−fold] | Yield [%] |
|---|---|---|---|---|---|
|
PMO-01867 |
|
|
|
|
|
| Culture supernatant (2.5 L) |
3.935 |
1.48 |
0.38 |
1 |
100 |
| Phenyl Sepharose |
0.536 |
0.39 |
0.74 |
2.0 |
27 |
| Q-Source |
0.211 |
0.25 |
1.18 |
3.2 |
17 |
| Gel filtration |
0.060 |
0.12 |
1.91 |
5.1 |
7.8 |
|
PMO-02916 |
|
|
|
|
|
| Culture supernatant (2.1 L) |
5.800 |
9.21 |
1.59 |
1 |
100 |
| Phenyl Sepharose |
0.334 |
1.10 |
3.28 |
2.1 |
12 |
| Q-Source |
0.140 |
0.78 |
5.57 |
3.5 |
8 |
|
PMO-03328 |
|
|
|
|
|
| Culture supernatant (2.9 L) |
5.275 |
2.08 |
0.39 |
1 |
100 |
| Phenyl Sepharose |
0.235 |
1.57 |
6.70 |
17.0 |
76 |
| Q-Source |
0.074 |
0.70 |
9.52 |
24.1 |
34 |
| Gel filtration |
0.007 |
0.11 |
15.76 |
39.9 |
5.4 |
|
PMO-08760 |
|
|
|
|
|
| Culture supernatant (2.3 L) |
3.052 |
3.19 |
1.05 |
1 |
100 |
| Phenyl Sepharose |
0.158 |
0.30 |
1.87 |
1.8 |
9 |
| Q-Source |
0.047 |
0.12 |
2.54 |
2.4 |
4 |
| Gel filtration | 0.005 | 0.02 | 4.08 | 3.9 | 0.6 |
aAll protein concentrations were measured with the Bradford assay.