Table 1. Fluorescent Protein Excitation and Emission.

Donor FP	Acceptor FP	Sensor Name	Excitation max (nm)	Emission max (nm)
CFP	YFP	ZapCY2	435	535
tSapphire	mKO	ZapSM2	399	559
tSapphire	TagRFP	ZapSR2	399	580
mOrange2	mCherry	ZapOC2	549	610
mOrange2	mK ATE	ZapOK2	549	633
Clover	mRuby2	ZapCmR1	486	605
Clover	mRuby2	ZapCmR1.1	486	605
Clover	mRuby2	ZapCmR2	486	605

Senor nomenclature is as follows: Zap refers to the 1^st two zinc fingers of the Saccaromyces cerevisiae Zap1 transcription factor that serves as the zinc binding domain, the next two letters refer to the donor and acceptor FPs, finally the “1” at the end of a sensor name indicates the wild type Zap1 domain was used, the “2” indicates that two mutations (Cys to His) were incorporated to lower the zinc affinity, as outlined in [15]. “1.1” indicates one mutation (Cys to His) was incorporated.