Figure 3. smg-8 lacks an NMD phenotype for the native NMD target rpl-12.

(A) RT-PCR was performed using a pair of primers that distinguish the two spliced isoforms of rpl-12; the upper, PTC band is visible only when the NMD pathway is compromised by smg-1, smg-2 or smg-3 mutations (lanes 2, 3 and 4). Only the lower, WT band is observed in wild-type (lane 1) and smg-8 mutant (lane 5) animals. (B) Wild-type worms were fed bacteria expressing dsRNA targeting smg-1, smg-8 or smg-9 from the Ahringer dsRNA library [26]. RNA was analyzed as in (A). (C) As in (B), using an enhanced RNAi mutant eri-6/7 [22], [23]. (D) As in (B), using the smg-8(tm2937) mutant strain.