Figure 5. smg-8 does not restore expression of myo-3::GFP, an exogenous NMD target.

(A) Schematic representation of the exogenous NMD GFP reporter, driven by the myo-3 promoter, which is destabilized by an amino acid sequence that marks a protein for degradation (degron), and a long 3′UTR [24]. (B) smg-8 and control mutations were introduced into CL724 (myo-3::GFP) worms. The double combinations were then inspected under a fluorescent microscope. smg-1 and smg-3 mutants express high levels of GFP. In contrast, smg-8 animals photographed under the same conditions show only a slight accumulation of GFP, similar to the wild type.