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. 2012 Nov 16;7(11):e49589. doi: 10.1371/journal.pone.0049589

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Schematic representation of fusion proteins with three different N-terminal tags: H₆, H₆-GST, and H₆-MBP (not to scale). In the tagged forms of TEV protease, the canonical TEV protease recognition site (ENLYFQG) was replaced by an uncleavable recognition site ENLYFQP [21] to prevent autodigestion of the fusion proteins.