Figure 4. Interaction of MBP fusion proteins with GroEL/S.

(A) Lysed cells co-expressing H₆-MBP-GFP and either wild-type GroE or the GroE_3–1 variant are shown under blue or white light illumination. Cells co-expressing GroE_3–1 fluoresce more intensely than cells co-expressing wild-type GroE as a result of enhanced GFP folding. Cells expressing only the MBP-GFP fusion protein are shown on the left. (B) SDS-PAGE analysis of total and soluble proteins from the cells in (A). T, total intracellular protein; S, soluble intracellular protein.