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. 2012 Nov 16;7(11):e49589. doi: 10.1371/journal.pone.0049589

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Expression and solubility of wild type MBP (MBP_wt) and mutant MBP (I329W) fusion proteins are shown in the figure. The co-expression of GroEL/S along with mutant MBP fusions rescues the solubility (right most pair of lanes). The passenger proteins were GFP (top), E6 (middle) and p16 (bottom). A Western blot using anti-His₆ tag antibody is shown to the right since the fusion proteins and GroEL co-migrates in the case of E6 and p16 (MBP fusion proteins carry a His₆ tag at the N-terminus); loading is similar to the respective gels on the left.