Figure 2. Short run gel electrophoresis used for sample preparation and in-gel digestion.

A 12.5% polyacrylamide gel was used to immobilize the samples and facilitate a highly reproducible in-gel digestion. Each lane contains 20 µg total protein. The gel was run at 150 V for 15 min to give a length of approximately 1.5 cm. After fixing, the gel was stained for 5 min. The staining pattern shows equal loading of each sample. Each lane was then cut as a single sample for processing via a typical in-gel digestion protocol. The treatment groups in this gel are: 1, control; 2 −+oxLDL; 3+Nrf2 siRNA; 4+both. Each treatment was n = 5 to give a set of 20 samples making two gels necessary.