Table 2. Housekeeping proteins monitored to demonstrate equal loading of the gel and consistent responses in the LC-tandem MS analysis.
Gene name | Protein name | Molecular weight (kDa) | Function | |
1 | Calr | calreticulin | 53 | endoplasmic reticulum calcium binding protein |
2 | Gapdh | glyceraldehyde-3-phosphate dehydrogenase | 36 | cytosolic enzyme in carbohydrate metabolism |
3 | Hspd1 | 60 kDa heat shock protein, mitochondrial | 58 | mitochondrial chaperonin |
4 | Ldha | lactate dehydrogenase A | 36 | cytoplasmic enzyme in anaerobic glycolysis |
5 | Lyz | chicken lysozyme | 16 | non-endogenous internal standard |
6 | Ncl | nucleolin | 76 | nuclear RNA and DNA binding protein |
7 | Rps8 | 40S ribosomal protein S8 | 24 | structural constituent of the ribosome |