Table 3. In-run precision.
Full assay monitoring 317 fragmentation reactions covering 35 proteins | truncated assay monitoring 98 fragmentation reactions covering 10 proteins | |
Gene name | Relative standard deviation | Relative standard deviation |
Prdx1 | 5.6% | 3.1% |
Ldha | 3.6% | 2.0% |
Txn1 | 4.7% | 3.1% |
Hspd1 | 5.5% | 4.4% |
Cat | 6.4% | 1.5% |
Akr1b1 | 9.1% | 4.7% |
G6pd | 7.0% | 5.3% |
Sod1 | 10.3% | 2.8% |
Txnrd1 | 8.2% | 3.5% |
Sod2 | 7.8% | 2.8% |
average for 10 selected proteins = 6.8%average for all 36 detectableproteins = 7.2% | average for 10 selected proteins = 3.3% |