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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: Cell. 2012 Aug 23;150(5):1002–1015. doi: 10.1016/j.cell.2012.07.017

Figure 2. RBR-SCR Interaction Affects the Expression of SCR-SHR Direct Targets.

Figure 2

(A–C) pMGP::MGP:GFP protein fusion expression in WT (A) and inducible RBR overexpressor line before (B) and after transfer for 24 hr (C) to Dex-amethazone (hpDex).

(D–F) pCYCD6;1::GFP fusion expression in WT (D) and in scr4-1;SCRAxCxA (E); note the correlation between expansion of pCYCD6;1::GFP expression domain and appearance of extra ACDs, marked with asterisks; asymmetric segregation of pSCR::SCRAxCxA:YFP in the same plant (F).

(G) ChIP-qPCR using RBR antibody and primers to specific regions of NUC, PCNA, and IR1 promoters. Black asterisks, Student’s t test (p < 0.05); fragment −2.2 showed variable enrichment in biological replicates.