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Specificity of purified elm seed PLA2
| Substrate | Recovered
14C Radioactivity
|
|
|---|---|---|
| Fatty acids | LPC | |
| % of total | ||
| 1-Palmitoyl-2-[14C]palmitoyl-PC | 48 | 0.3 |
| 1,2-[14C]Dipalmitoyl-PC | 34 | 30 |
| 1-Palmitoyl-2-[14C]palmitoyl-DAG | 0 | – |
| 1-[14C]Oleoyl-LPC | 0.2 | 99.8 |
Purified elm PLA2, 2 ng, was incubated for 30 min with 5 nmol of radioactive substrate under standard assay conditions.
