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Acyl specificities of the purified elm seed and cobra PLA2s
| Substrate | PLA2 Activity
|
|
|---|---|---|
| Elm PLA2 | Cobra PLA2 | |
| pmol/min | ||
| 1-Palmitoyl-2-[14C]palmitoyl-PC | 39 | 48 |
| 1-Palmitoyl-2-[14C]oleoyl-PC | 77 | 40 |
| 1-Palmitoyl-2-[14C]caproyl-PC | 93 | 51 |
Purified elm or cobra PLA2, 1 ng, was incubated for 10 min with 25 nmol of radioactive substrate under standard assay conditions.
