Figure 2. Illustration of co-registration between optical images and pathology.

Co-registration of spectroscopic images with pathology in large, fresh tissue sections. (a) Slice of a fresh lumpectomy section inked on its margins. A thin paraffin window is placed between the tissue surface and glass plate during imaging to locate the imaged field in an inverted geometry. When the tissue is removed from the plate, inked pins are placed at the corners of the imaged field. (b) The imaged region is marked with inked pins and the tissue is fixed in formalin with pins in place. (c) Fixed tissue was paraffin embedded and processed for histology. (d–e) Pathology correlates were determined within areas bound by the pin marks indicated by the blue arrows.