Figure 4. Diagnostic performance of spectral parameters.

Box plots of fitted scattering parameters as a function of diagnosis: (a) mean scattering slope per 1×1cm² FOV, (b) mean integrated irradiance per 1×1cm² FOV. The green star in (a) is an indicator of the invasive cancer extreme (see text for further details). (c) ROC analysis of threshold-based diagnosis of benign and malignant pathologies according to region averaged scattering slope as a function of region size (bin length). (d) Area under the curve as a function of region size revealed that 10×10 spectra, or spectra sampled within a 1×1mm² area, sufficiently accounted for biological variance and produced a robust diagnosis.