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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Cancer Res. 2012 Aug 31;72(22):5833–5842. doi: 10.1158/0008-5472.CAN-12-1229

Figure 5. RGS4 inhibits the proliferation, migration, VEGF secretion, and Akt activation of vGPCR-expressing cells.

Figure 5

(A) Expression of RGS4 resulted in a strong inhibition of proliferation of the vGPCR-expressing SVECs. vGPCR/CTR and vGPCR/RGS4 are pooled populations of SVEC/vGPCR cells that were stably transfected with a control and an RGS4-expressing vector, respectively. (B) Expression of RGS4 reduced cell migration of vGPCR-expressing cells in the presence or absence of Gro-α. (C) RGS4 inhibited the secretion of vascular endothelial growth factor (VEGF) by the vGPCR-expressing cells based on ELISA. (D) Stable expression of RGS4 inhibited Gro-α-induced phosphorylation of Akt (S473) in the vGPCR-expressing cells. The outcome of proliferation and migration assays are displayed as a percent average of cell number/migration ± standard deviation. *, p <0. 05; **, p <0. 01. Each experiment was performed twice with consistent results.