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. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: Am J Surg Pathol. 2012 Dec;36(12):1826–1834. doi: 10.1097/PAS.0b013e31825ec07a

Fig. 2 — Comparison of immunoreactivity of p53 and laminin γ1 in a fallopian tube diffusely involved by a serous tubal intraepithelial carcinoma. A. Western blot analysis shows a marked decrease in lamininγ1 protein expression after OVCAR3 cells were treated with siRNA-1 and siRNA-2 that target LAMC1 gene. GAPDH is used as a loading control. B. Immunostaining of laminin γ1, p53 and Ki-67 in a STIC. A high magnification view of a STIC and adjacent normal-appearing tubal epithelium. The STIC demonstrates a diffuse and intense p53 staining, a high Ki-67 labeling index and diffuse of laminin γ1 expression. In normal tubal epithelium, of lamininγ1 immunoreactivity is detected in basement membrane and apical surface of ciliated cells (inset) whereas of laminin γ1 immunoreactivity in STIC cells loses the “polarity” with strong staining in both cell surface and cytoplasm. C. A low magnification view of a fallopian tube section contains diffuse STIC. The distribution of laminin γ1 immunoreactivity faithfully overlapped with p53 staining. Asterisks highlight the discrete areas of tubal epithelium that is negative for both stains.

Fig. 2 — Comparison of immunoreactivity of p53 and laminin γ1 in a fallopian tube diffusely involved by a serous tubal intraepithelial carcinoma. A. Western blot analysis shows a marked decrease in lamininγ1 protein expression after OVCAR3 cells were treated with siRNA-1 and siRNA-2 that target LAMC1 gene. GAPDH is used as a loading control. B. Immunostaining of laminin γ1, p53 and Ki-67 in a STIC. A high magnification view of a STIC and adjacent normal-appearing tubal epithelium. The STIC demonstrates a diffuse and intense p53 staining, a high Ki-67 labeling index and diffuse of laminin γ1 expression. In normal tubal epithelium, of lamininγ1 immunoreactivity is detected in basement membrane and apical surface of ciliated cells (inset) whereas of laminin γ1 immunoreactivity in STIC cells loses the “polarity” with strong staining in both cell surface and cytoplasm. C. A low magnification view of a fallopian tube section contains diffuse STIC. The distribution of laminin γ1 immunoreactivity faithfully overlapped with p53 staining. Asterisks highlight the discrete areas of tubal epithelium that is negative for both stains.