Figure 3. IEC-specific overexpression of PHB is associated with altered levels of p53, PUMA, Bax and activation of Bad.

Representative Western blots of total protein isolated from colonic mucosal strippings were probed with (A) anti-PHB, (B) anti-p53, (C) anti-PUMA, (D) anti-Bax, and (E) anti-phospho-Bad and anti-Bad. Anti-β-actin was used as a loading control. Histograms show cumulative mean band densitometry ± SEM, respectively. n ≥ 5 per treatment. *P < 0.05, **P < 0.01.