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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Cancer Res. 2012 Aug 6;72(22):5778–5789. doi: 10.1158/0008-5472.CAN-12-0603

Figure 5. PHB interacts with phospho-STAT3 and modulates STAT3 responsive genes, Bcl-xL and Bcl-2 in vivo and in vitro.

Figure 5

(A) Representative Western blots of pSTAT3, Bcl-2, Bcl-xL protein expression in Caco2-BBE cells stably overexpressing pEGFPN1-PHB (GFP-PHB) or empty vector and treated with 100 ng/ml IL-6 basolaterally for 1 hour. Blots were subsequently probed with anti-GFP to ensure transfection efficiency. (B-C) Co-immunoprecipitation of PHB and pSTAT3 using cell lysates described in (A). (D) PHB was immunoprecipitated from colon mucosal protein lysates from water- and AOM DSS-treated mice, followed by immunoblot for pSTAT3 expression. (E) Representative Western blots of total protein isolated from colonic mucosal strippings were probed with anti-Bcl-2, anti-Bcl-xL, anti-β-actin (loading control). Histograms show mean band densitometry ± SEM. n ≥ 5 per treatment. *P < 0.05,