Figure 1. ROR1 is a functional target in t(1;19) ALL.

White Blood cells (2.25 × 10⁷) from a t(1;19)-positive ALL patient (07-112) were suspended in siPORT buffer and incubated with 1 µM siRNA from an siRNA library individually targeting each member of the tyrosine kinase family as well as N-RAS, K-RAS, and single and pooled non-specific siRNA controls. Cells were electroporated on a 96-well electroporation plate at 1110 V (equivalent of 150 V), 200 µsec, 2 pulses. Cells were replated into culture media and cell viability was determined by addition of a tetrazolium salt (MTS assay) at day 4 post-electroporation. Values represent percent mean (normalized to the median value on the plate) ± s.e.m (n = 3). See also Figure S1.