TABLE 3.

The effect of hBChE variants on kinetic parameters

Data were fit to the Webb equation (AKA modified Haldane equation) where B = 1 yields the Michaelis-Menten equation.

		WT	G117R	G117H	G117N	E197C	L125V
BTC	Km, μM	23 ± 12	490 ± 130	220 ± 10	900 ± 100	210 ± 40	1500 ± 100
	B	3.7 ± 0.7	1.3 ± 0.15	2.2	1*	1*	1*
	Kss, mM	0.74 ± 0.09	1	0.55 ± 0.06	N.A.	4.4 ± 0.7	N.A.
ATC	Km, μM	25 ± 17	600 ± 30	1200 ± 40	190 ± 90	91 ± 5	80 ± 20
	B	5 ± 2	1	2.7	5.7 ± 0.1	3.1 ± 0.1	3.1 ± 0.1
	Kss, mM	2.2 ± 0.6	N.A.	1.2 ± 0.2	12 ± 1	9 ± 1	9 ± 1
ETP	ki, min−1·μM−1	2.0 ± 0.1	<0.02	<0.02	<0.02	1.5 ± 0.1	0.083 ± 0.004
Paraoxon	ki, min−1·μM−1	1.2 ± 0.1	0.19 ± 0.01	<0.01	<0.01	0.7 ± 0.1	0.07 ± 0.01
SpGD	ki, min−1·μM−1	0.20 ± 0.01	∼0.001	<0.001	<0.001	0.076 ± 0.005	0.012 ± 0.004
SpGB	ki, min−1·μM−1	0.12 ± 0.01	<0.004	<0.004	<0.004	0.22 ± 0.01	(0.007 ± 0.003)
SpGAc	ki, min−1·μM−1	26 ± 1	<1	<1	<1	16 ± 1	1.8 ± 0.3
SpGF	ki, min−1·μM−1	2.4 ± 0.1	<0.05	<0.05	<0.05	0.94 ± 0.02	0.15 ± 0.01

N.A., not available.

^*, value was assigned as a constant before analysis.