Fig. 7.

CID-2745687 and ML-145 both block agonist-mediated internalization of human but not rodent forms of GPR35. A to C, the ability of varying concentrations of zaprinast (A), cromolyn disodium (B), and pamoate (C) to promote internalization of human GPR35-eYFP and the capacity of either CID-2745687 (○) or ML-145 (▴) (1 × 10 ⁻⁵ M) to prevent this was assessed via high content imaging using an ArrayScan high content imager. D and E, in equivalent studies the capacity of varying concentrations of zaprinast to cause internalization of rat (D) and mouse (E) GPR35-eYFP and any capacity of CID-2745687 and ML-145 (1 × 10⁻⁵ M) to prevent this was assessed.