Fig. 9.
GPR35 stimulates IP1 accumulation when coexpressed with suitable chimeric G protein α subunits. A, HEK293T cells were transfected with or without human FLAG-GPR35-eYFP alongside either Gqi5 or Gq135 Gα subunits. Basal (open bars) IP1 accumulation and effects of zaprinast (1 × 10 −5 M) on IP1 accumulation (filled bars) were measured. A positive control was provided after transfection of the M3 muscarinic acetylcholine receptor and addition of the agonist carbachol (1 × 10 −3 M). B, after cotransfection of human FLAG-GPR35-eYFP with either Gqi5 (●) or Gq135 (■) Gα subunits the effect of various concentrations of zaprinast were assessed. C, after transfection of human (●), mouse (■), or rat (▴) FLAG-GPR35-eYFP with Gq135 the ability of varying concentrations of zaprinast (left), pamoate (center), or cromolyn disodium (right) to promote IP1 accumulation was assessed.