Fig. 1.

A novel chromatographic method for the separation of maraviroc oxidative metabolites. A, chemical structure of maraviroc and sites of oxidative metabolism (Abel et al., 2008a). Separation of oxidative metabolites from human liver microsomal reactions (B), human plasma (C), and human urine (D). The human plasma and urine chromatograms are representative of data obtained 2 and 2 to 4 h, respectively, after a single oral dose of 300 mg of maraviroc. Metabolites were analyzed by mass spectrometry using the transitions m/z 530 → 296 for M1 to M5 and 530 → 280 for M6 after separation on an UPLC column.