Fig. 3.

M1 formation is decreased in human liver microsomes genotyped as CYP3A5*3/*3, and M1 is the major oxidative metabolite produced by CYP3A5*1/*1 human liver microsomes. A, reduced M1 formation by human liver microsomes homozygous for a CYP3A5 nonfunctional allele. Human liver microsomes from individuals genotyped as CYP3A5*1/*1 or CYP3A5*3/*3 were incubated with 2 μM maraviroc and M1 formation was measured. Each data point represents the peak area ratio of M1 to maraviroc-d₆ (internal standard). The mean for each group is indicated by a horizontal line. B and C, representative chromatogram of oxidative metabolites from reactions of CYP3A5*1/*1 human liver microsomes (B) and CYP3A5*3/*3 human liver microsomes (C).