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ESI-LC-MS analysis of CYP2B6 after incubation with tamoxifen. CYP2B6 was incubated with 60 μM tamoxifen in the presence and absence of NADPH. The incubation conditions and analyses are described under Materials and Methods. The figure shows the deconvoluted mass spectrum of the modified (54,761 Da) and the unmodified CYP2B6 apoprotein (54,373 Da). The inset shows the deconvoluted spectrum of the apo2B6 from a control incubation in the presence of tamoxifen and in the absence of NADPH.
