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. 2012 Dec;40(12):2280–2288. doi: 10.1124/dmd.112.047266

Fig. 6.

Fig. 6.

LC-MS/MS spectrum of the CYP2B6 tryptic peptide modified by tamoxifen. CYP2B6 was reconstituted with reductase and tamoxifen in the presence or absence of NADPH and digested with trypsin. The samples were analyzed by LC-MS/MS and Sequest software as described under Materials and Methods. The figure shows the profile for the doubly charged ion with m/z 678.2 for the modified peptide 188FHYQDQE194. The inset shows the calculated b and y ions for the modified peptide, and the b and y ions identified in the MS/MS spectrum are shown in blue and red, respectively.