Rapamycin pretreatment during conditioning does not affect the acquisition of a cocaine-induced place preference or locomotor sensitization. (a) Schematic of experimental design. Mice received IP DMSO vehicle (n=12) or 10 mg/kg rapamycin (n=12) 1 hr prior to each cocaine/saline pairing, followed by a drug-free posttest (#1), a rapamycin-pretreated posttest (#2), a 21-day withdrawal period, and a test for the expression of cocaine-induced locomotor sensitization, following 1-hr rapamycin or vehicle pretreatment. (b) Rapamycin pretreatment during place-conditioning procedures had no effect on locomotor activity elicited by cocaine or by saline. (c) Rapamycin pretreatment during place-conditioning procedures also did not block a conditioned place-preference when animals were tested 24 hrs later in a drug-free state. Data are expressed as means ± SEM (error bars). *p<0.05 vs. saline-paired compartment (i.e., place-preference).