Figure 3.
Changes in Sod4 and Sod4A transcript accumulation in 28-dpp developing embryos in the presence of ABA. Embryos were isolated from 28-dpp kernels of greenhouse-grown W64A plants and incubated on Murashige-Skoog medium supplemented with either increasing concentrations of ABA for 24 h (top) or with (+)/without (−) 10−4 m ABA in the dark for 0, 2, 4, 8, 12, and 24 h (bottom). Scutella were isolated for RNA isolation. Total RNA (10 μg) was used for northern-blot analysis. Blot was probed with the Sod4, Sod4A gene-specific probes, Em, and 18S rDNA. ip, In planta control (0 h of treatment).