Figure 4.
Changes in Sod4 and Sod4A transcript accumulation the presence of ABA in postgermination embryos. Embryos were excised from 5-dpi germinating W64A seeds and treated with either increasing concentrations of ABA for 24 h (top) or with 0 (−) and 10−3 m ABA (+) for 2, 4, 12, and 24 h (bottom). Scutella were isolated from treated embryos and used for RNA isolation and northern-blot analysis. Total RNA (10 μg) was used for northern blot and probed with Sod4 and Sod4A gene-specific probes. The 18S rRNA was used as a loading control. ip, In planta control (0 h of treatment).