Figure 5.
Accumulation of the Sod4 and Sod4A transcripts in response to high osmoticum in developing and germinating embryos. Top, Embryos were dissected from developing kernels of W64A 28 dpp and incubated on Murashige-Skoog medium in the dark for 0, 2, 4, 8, 12, and 24 h with (+) or without (−) mannitol (11%, w/v). Bottom, Embryos were excised from 5-dpi germinating W64A seeds and treated with 20% (w/v) Suc, 11% (w/v) mannitol, or 10−4 m ABA for 24 h in the dark. Scutella were isolated and used for RNA isolation and northern-blot analysis. Total RNA (10 μg) was separated on 1.2% denaturing gel and transferred to a nylon membrane. The blot was sequentially hybridized with Sod4, Sod4A, Em, and 18S probes.