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Changes in Sod4 and Sod4A transcript accumulation in response to 10−4 m ABA in 18-dpp vp1 embryos and their wild-type sibling. Embryos were collected from mutant (vp1/vp1) and wild type (Vp1/−) at 18 dpp and treated with or without 10−4 m ABA for 24 h. Total RNA (10 μg) was used for northern-blot analysis and probed with Sod4 and Sod4A gene-specific probe, as well as the Em of wheat. The 18S rRNA was used as a loading and transfer control.
