Figure 8.
Kinetics of accumulation of the Sod4 and the Sod4A transcripts in response to ABA and osmotic stress in 7-d-old leaves. Seven-day-old young W64A seedlings were harvested and roots were soaked with 10−4 m ABA solution or 11% mannitol solution for 2, 4, 8, 12, and 24 h with constant light. Total RNA was isolated from leaves and 20 μg of RNA from each sample was used for northern-blot analysis and probed with Sod4, Sod4A, and Cat1 gene-specific probes. The 18S rDNA was used as a loading control.