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. Author manuscript; available in PMC: 2012 Nov 18.
Published in final edited form as: Neuroimage. 2011 Jan 31;55(4):1461–1474. doi: 10.1016/j.neuroimage.2011.01.064

Figure 4.

Figure 4

Presynaptic characteristics of ACC (area 32) projections to MTL parahippocampal cortices. LM Analysis: A, B, Bouton major diameter frequency distributions in the superficial (A) and deep (B) layers of area TF following 2D analysis at the light microscope. Insets show the range of the distribution. C, Bouton major diameter clusters in area TF following 2D analysis at the light microscope. The average major diameter for distributions in the superficial and deep layers was equal to 1.0 μm (denoted by x). D, The ratio of large to small boutons, based on the values determined from the cluster analysis approached 50:50 in the superficial and deep layers of parahippocampal area TF following 2D analyses at the LM. E, Large and small boutons were about evenly distributed within the superficial and deep layers of parahippocampal areas TH and TF quantified using a combination of exhaustive sampling and stereological techniques. F, Relative densities of labeled boutons collapsed across parahippocampal areas. The even distribution of small and large boutons following exhaustive and stereological quantification at the LM is very similar to that observed following the 2D analysis at the LM (D). Abbreviations: ACC: anterior cingulate cortex; D: deep layers; LM: light microscope; U: upper layers.