TABLE 4.

Output file names and description of contents.

File name	Description
fitout_glblparams.txt	Best-fit global parameters and s.d. (σ) for each parameter equal to 68% confidence interval
fitted_R2dark1.txt, fitted_R2dark2.txt	Residue-specific R2 and σ (R2) (s−1) of the dark state(s). Both are computed for fit type 3–7, and only the R2 in the single dark state is computed for fit type 1
fitted_R2tethered.txt	Residue-specific R2 for tethered state. Computed for fit type 0 and 1
fitted_K3.txt	Residue-specific equilibrium constant K3 = [direct-contact]/[tethered]. Computed for fit type 0 or 1
plotres_[X].png	Plot of the DEST data as a function of saturation pulse offset frequency for residue [X], in .png image file format
plotvarnum_[Y].png	Plot of residue-specific parameter [Y] as a function of residue. Varnum 1 and 2 correspond to $R_2^{\text{tethered}}$ and K3, respectively, for fit types 0 and 1, and to R2 in dark states 1 and 2, $R_2^{\text{dark}(1)}$ and $R_2^{\text{dark}(2)}$, respectively, for fit types 2 to 7
plot_deltaR2.png	Plot of ΔR2 experimental versus that computed from best-fit model, in .png image file format
exptDEST_res[X].txt	Experimental input DEST data for residue [X], formatted for ease of plotting. Format: saturation offset, relative peak intensity as a function of offset and saturation field (Hz)
fitDEST_res[X].txt	Best-fit model DEST data for residue [X], formatted for ease of plotting. Format: saturation offset, relative peak intensity as a function of offset and saturation field (Hz)