Table 1.

Proteins interacting with human RECQL4 and its homolog

Protein1	Location	Detection methods2	Interaction region on RECQL4	Interaction function	References
Cut5	ND	CoIP	ND	DNA replication	[28]
MCM10	Nuclear	CoIP, MS, GST-PD	1–200aa	RECQL4 helicase inhibition	[38]
RAD51	Nuclear	CoLC, CoIP	ND	DNA double strand break repair	[15]
PARP1	ND	PDS, CoIP	833–1208aa	Base excision repair	[16]
XPA	Nuclear	CoIP, GST- PD, CoLC, CoFC	ND	Nucleotide excision repair	[18]
FEN1	Nuclear	CoIP, CoLC	ND	FEN1 incision stimulation	[19]
POLβ	Nuclear	CoLC	ND	DNA Polβ primer extension activity stimulation	[19]
APE1	Nuclear	CoLC	ND	APE1 endonuclease stimulation	[19]
RPA	Nucleus	CoLC	ND	RecQL4 helicase stimulation	[33]
BLM	Nuclear	CoIP, Y2H	1–471 aa	BLM stimulation	[43]
URB1/2	ND	CoIP, CoFC, MS	ND	Unknown	[17]
p300	Nuclear	CoIP, GST- PD, CoLC	1– 408 aa	Regulation of cellular location of RECQL4	[31]
p53	Mitochondria	CoLC, CoFC, CoIP	270–400 aa	Silencing of nuclear location signals of RECQL4 and p53	[22]
TOM20	Mitochondria	GST-PD	13–18 aa	Import into mitochondria	[22]
TFAM	Mitochondria	CoIP	ND	Unknown	[21]
TRF1	Nuclear/telomere	CoLC,	ND	RECQL4 helicase stimulation and telomere maintenance	[37]
TRF2	Nuclear/telomere	CoIP	ND	RECQL4 helicase stimulation and telomere maintenance	[37]
WRN	Nuclear/telomere	CoIP	ND	WRN stimulation on telomeric D-loop	[37]

¹All RecQL4 interacting partners were found in human cells except cut5, which is a xenopus protein;

²Abbreviation of detection methods as follows: ND, not determined; CoIP, co-immunoprecipitation; MS, mass spectrometry; GST-PD, GST pull-down assay; CoLC, Co-localization; CoFC, Co-fractionation; PDS, phage display screen; Y2H, yeast two hybrid assay; aa, amino acid.